网站首页 | 公司介绍 | 产品中心 | 资料下载 | 常见问题 | 成功案例 | 客户服务 | 联系我们
办事处简介
关于 CTL
关于 Paul
技术优势
客户列表
研究论著
主页> 关于我们 > 公司介绍  
　　
　　美国 CTL 公司创始人 Dr. Paul V. Lehmann 是一位国际著名的免疫学专家。截至 2003 年底， Dr. Paul V. Lehmann 及其领导下的研究人员一共在国际权威刊物发表 56 篇文章。为了方便科研客户检索，兹列于其下。
Single-Cell Resolution/Exact Frequency Measurements of Antigen-Specific T cells Ex Vivo.
  • Helms, T., B.O. Boehm, R. J. Assad, R.T. Trezza, P.V. Lehmann, and M. Tary-Lehmann. 2000. Direct visualization of cytokine-producing, recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients. J. Immunol. 164: 3723-3732.
  • Karulin, A.Y., M.D. Hesse, M. Tary-Lehmann, and P.V. Lehmann. 2000. Single-cytokine-producing CD4 memory cells prevail in vivo, in type 1/type 2 immunity. J. Immunol. 164:1862-1872.
  • Hesse, M.D., A.Y. Karulin, B.O. Boehm, P.V. Lehmann, and M. Tary-Lehmann 2001. A T cell clone's avidity is a function of its activation state. J. Immunol. 167: 1353-1361.
  • Targoni, O.S., J. Baus, H.H. Hoffstetter , M.D. Hesse, A.Y. Karulin, B.O. Boehm, T. Forsthuber, and P.V. Lehmann. 2001. Frequencies of neuroantigen-specific T cells in the CNS versus the immune periphery during the course of EAE. J. Immunol. 166: 4757-4764.
Measuring Type 1/Type 2 Immunity
  • Helms, T., B.O. Boehm, R. J. Assad, R.T. Trezza, P.V. Lehmann, and M. Tary-Lehmann. 2000. Direct visualization of cytokine-producing, recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients. J. Immunol. 164: 3723-3732.
  • Forsthuber, T., H.C. Yip, and P.V. Lehmann. 1996. Induction of TH1 and TH2 immunity in neonatal mice. Science. 271: 1728-1730.
  • Chu, R.S., O.S. Targoni, A.M. Krieg, P.V. Lehmann, and C.V. Harding. 1997. CpG oligodeoxynucleotides act as adjuvants that switch on T helper 1 (Th1) immunity. J. Exp. Med. 186: 1623-1631.
  • Yip, H.C., A.Y. Karulin, M. Tary-Lehmann, M.D. Hesse, H. Radeke, P. Heeger, R.T. Trezza, F.P. Heinzel, T. Forsthuber, and P.V. Lehmann. 1999. Adjuvant guided type 1 and type 2 immunity: Infectious/non-infectious dichotomy defines the class of response. J. Immunol. 162: 3942-3949.
  • Karulin, A.Y., M.D. Hesse, H.C. Yip, and P.V. Lehmann. 2002. Indirect IL-4 pathway in Th1 immunity. J. Immunol. 168: 545-553.
  • Guerkov, R.E., O.S. Targoni, C.R. Kreher, B.O. Boehm, M.T. Herrera, M. Tary-Lehmann, P.V. Lehmann, and S.K. Schwander. 2003. Detection of low-frequency antigen-specific IL-10-producing CD4(+) T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine. J. Immunol. Methods. 279: 111-121.
Cognate Cytokine Production by T Cells vs Bystander Cytokine Production
  • Helms, T., B.O. Boehm, R. J. Assad, R.T. Trezza, P.V. Lehmann, and M. Tary-Lehmann. 2000. Direct visualization of cytokine-producing, recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients. J. Immunol. 164: 3723-3732.
  • Karulin, A.Y., M.D. Hesse, M. Tary-Lehmann, and P.V. Lehmann. 2000. Single-cytokine-producing CD4 memory cells prevail in vivo, in type 1/type 2 immunity. J. Immunol. 164:1862-1872.
  • Karulin, A.Y., M.D. Hesse, H.C. Yip, and P.V. Lehmann. 2002. Indirect IL-4 pathway in Th1 immunity. J. Immunol. 168: 545-553.
  • Guerkov, R.E., O.S. Targoni, C.R. Kreher, B.O. Boehm, M.T. Herrera, M. Tary-Lehmann, P.V. Lehmann, and S.K. Schwander. 2003. Detection of low-frequency antigen-specific IL-10-producing CD4(+) T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine. J. Immunol. Methods. 279: 111-121.
Measurements of Functional T Cell Avidity
  • Hesse, M.D., A.Y. Karulin, B.O. Boehm, P.V. Lehmann, and M. Tary-Lehmann. 2001. A T cell clone's avidity is a function of its activation state. J. Immunol. 167: 1353-1361.
  • Targoni, O.S. and P.V. Lehmann. 1998. Endogenous myelin basic protein inactivates the high avidity T cell repertoire. J. Exp. Med. 187: 2055-2063.
  • Yip, H.C., A.Y. Karulin, M. Tary-Lehmann, M.D. Hesse, H. Radeke, P. Heeger, R.T. Trezza, F.P. Heinzel, T. Forsthuber, and P.V. Lehmann. 1999. Adjuvant guided type 1 and type 2 immunity: Infectious/non-infectious dichotomy defines the class of response. J. Immunol. 162: 3942-3949.
  • Karulin, A.Y., M.D. Hesse, M. Tary-Lehmann, and P.V. Lehmann. 2000. Single-cytokine-producing CD4 memory cells prevail in vivo, in type 1/type 2 immunity. J. Immunol. 164: 1862-1872.
Cytokine Co-Expression/Two Color ELISPOT
  • Karulin, A., M. Hesse, M. Tary-Lehmann, and P.V. Lehmann. 2000. Single-cytokine-producing CD4 memory cells prevail in vivo, in type 1/type 2 immunity. J. Immunol. 164: 1862-1872.
  • Heeger, P.S., T. Forsthuber, C. Shive, E. Beikert, C.P. Genain, H.H. Hofstetter, A.Y Karulin, and P.V. Lehmann. 2000. Revisiting tolerance induced by autoantigen in incomplete Freund's adjuvant. J. Immunol. 164: 5771-5781.
  • Karulin, A.Y., M.D. Hesse, H.C. Yip, and P.V. Lehmann. 2002. Indirect IL-4 pathway in Th1 immunity. J. Immunol. 168: 545-553.
Spot Size Distribution
  • Karulin, A.Y., M.D. Hesse, M. Tary-Lehmann, and P.V. Lehmann. 2000. Single-cytokine-producing CD4 memory cells prevail in vivo, in type 1/type 2 immunity. J. Immunol. 164: 1862-1872.
  • Hesse, M.D., A.Y. Karulin, B.O. Boehm, P.V. Lehmann, and M. Tary-Lehmann. 2001. A T cell clone's avidity is a function of its activation state.J. Immunol. 167: 1353-1361.
  • Guerkov, R.E., O.S. Targoni, C.R. Kreher, B.O. Boehm, M.T. Herrera, M. Tary-Lehmann, P.V. Lehmann, and S.K. Schwander. 2003. Detection of low-frequency antigen-specific IL-10-producing CD4(+) T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine. J. Immunol. Methods. 279: 111-121.
Determinant Mapping/High Throughput Screening
  • Targoni, O.S. and P.V. Lehmann. 1998. Endogenous myelin basic protein inactivates the high avidity T cell repertoire. J. Exp. Med. 187: 2055-2063.
  • Yip, H.C., A.Y. Karulin, M. Tary-Lehmann, M.D. Hesse, H. Radeke, P. Heeger, R.T. Trezza, F.P. Heinzel, T. Forsthuber, and P.V. Lehmann. 1999. Adjuvant guided type 1 and type 2 immunity: Infectious/ non-infectious dichotomy defines the class of response. J. Immunol. 162: 3942-3949.
  • Heeger, P.S., A. Valujskikh, and P.V. Lehmann. 2000. Comprehensive assessment of determinant specificity, frequency, and cytokine signature of the primed CD8 cell repertoire induced by a minor transplantation antigen. J. Immunol. 165: 1278-1284.
  • Pelfrey, C.M., R.A. Rudick, A.C. Cotleur, J.C. Lee, M. Tary-Lehmann, and P.V. Lehmann. 2000. Quantification of self-recognition in multiple sclerosis by single-cell analysis of cytokine production. J. Immunol. 165: 1641-1651.
  • Anthony, D.D., and P.V. Lehmann. 2003. T-cell epitope mapping using the ELISPOT approach. Methods. 29: 260-269.
Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC
  • Helms, T., B.O. Boehm, R.J. Assad, R.T. Trezza, P.V. Lehmann, and M. Tary-Lehmann. 2000. Direct visualization of cytokine-producing, recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients. J. Immunol. 164: 3723-3732.
  • Gebauer, B.S., D.E. Hricik, A. Atallah, K. Bryan, J. Riley, M. Tary-Lehmann, N.S. Greenspan, B.O. Boehm, B.J. Hering and P.S. Heeger. 2002. Evolution of the Enzyme-Linked Immunosorbent Spot Assay for Post-Transplant Alloreactivity as a Potentially Useful Immunme Monitoring Tool. The American Journal of Transplantation. 9: 857-866
  • Anthony, D.D., and P.V. Lehmann. 2003. T-cell epitope mapping using the ELISPOT approach. Methods. 29: 260-269.
  • Kreher, C.R., M.T. Dittrich, R. Guerkov, B.O. Boehm and M. Tary-Lehmann M. 2003. CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays. J. Immunol. Methods. 278: 79-93.
Fresh vs. Cryopreserved human PBMC
  • Gebauer, B.S., D.E. Hricik, A. Atallah, K. Bryan, J. Riley, M. Tary-Lehmann, N.S. Greenspan, B.O. Boehm, B.J. Hering and P.S. Heeger. 2002. Evolution of the Enzyme-Linked Immunosorbent Spot Assay for Post-Transplant Alloreactivity as a Potentially Useful Immune Monitoring Tool. The American Journal of Transplantation. 9: 857-866.
  • Kreher, C.R., M.T. Dittrich, R. Guerkov, B.O. Boehm and M. Tary-Lehmann M. 2003. CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays. J. Immunol. Methods. 278: 79-93.
CD8+ T cells and Granzyme B ELISPOT
  • Rininsland, F.H., T. Helms, R.J. Asaad, B.O. Boehm and M. Tary-Lehmann. 2000. Granzyme B ELISPOT assay for ex vivo measurement of T cell immunity. J. Immunol. Methods 240: 143-155.
  • Kleen, T.O., R. Assad, S. Landry, B.O. Boehm and M. Tary-Lehmann. 2003. Tc1 effector cell diversity in HIV infection: dissociated expression of IFN-? and of granzyme B by peptide-specific CD8 cells. AIDS, in press.
ELISPOT With Cells Other than Human PBMC, or Murine Lymph Node/Spleen Cells
  • Targoni, O.S., J. Baus, H.H. Hoffstetter , M.D. Hesse, A.Y. Karulin, B.O. Boehm, T. Forsthuber, and P.V. Lehmann. 2001. Frequencies of neuroantigen-specific T cells in the CNS versus the immune periphery during the course of EAE. J. Immunol. 166: 4757-4764. (lymphocytes isolated from murine CNS, peritoneal cavity)
  • Schwander, S.K., M. Torres, C.C. Carranza, D. Escobedo, M. Tary- Lehmann, P. Anderson, Z. Toossi, J.J. Ellner, and E. Sada. 2000. Pulmonary mononuclear cell responses to antigens of Mycobacterium tubercolosis in healthy houshold contacts of patients with active tuberculosis and healthy controls from the community. J. Immunol. 165: 1479-1485 (human bronchoalveolar lavage cells)
  • Gebauer, B.S., D.E. Hricik, A. Atallah, K. Bryan, J. Riley, M. Tary-Lehmann, N.S. Greenspan, B.O. Boehm, B.J. Hering and P.S. Heeger. 2002. Evolution of the Enzyme-Linked Immunosorbent Spot Assay for Post-Transplant Alloreactivity as a Potentially Useful Immune Monitoring Tool. The American Journal of Transplantation. 9: 857-866. (Human spleen cells, B cells and different APC subpopulations)
  • Aung, H., J. Sherman, M. Tary-Lehmann, Z. and Toossi. 2002. Analysis production of transforming growth factor-beta 1 (TGF-b1) expression in human monocytes infected with Mycrobacterium Avium at a single cell level by ELISPOT assay. J. Immunol. Methods 259: 25-32. (human macrophages)
Vaccine and Adjuvants
  • Forsthuber, T., H.C. Yip, and Lehmann P.V. 1996. Induction of TH1 and TH2 immunity in neonatal mice. Science, 271: 1728-1730.
  • Chu R.S., O.S. Targoni, A.M. Krieg, P.V. Lehmann, and C.V. Harding. 1997. CpG oligodeoxynucleotides act as adjuvants that switch on T helper 1 (Th1) immunity. J. Exp. Med. 186: 1623-163.
  • Heeger, P.S., T. Forsthuber, C. Shive, E. Beikert, C.P. Genain, H. Hofstetter, A. Karulin, and P.V. Lehmann. 2000. Revisiting tolerance induced by autoantigen in incomplete Freund's adjuvant. J. Immunol. 164: 5771-5781.
  • Gottwein J.M., T.G. Blanchard, O.A. Targoni, J.G.C. Eisenberg, B. M. Zagorski, R.V. Redline, J.G. Nedrud , M. Tary-Lehmann, P.V. Lehmann and S.J. Czinn. 2001. Protective anti-Helicobacter immunity is induced with aluminium hydroxide or complete Freud's adjuvant by systemic immunization. J. Infect. Dis. 184: 308-314.
  • Stern B.V., B.O. Boehm, M. Tary-Lehmann. 2002. Vaccination with tumor peptide in CpG adjuvant protects via IFN-? dependent CD4 cell immunity. J. Immunol. 168: 6099-6105.
  • Eisenberg, J., J.S. Czinn, C.A. Garhard, R.W. Redline, W.C. Bartholomae, J. Gottwein, J.G. Nedrud, S.E. Emancipator, B. O. Boehm, P.V. Lehmann, T.G. and Blanchard. 2003. Protective efficacy of anti-Helicobacter pylori immunity following systemic immunization of neonatal mice. Infection and Immunity, 71: 1820-1827.
Tumor Immunology
  • Stern, B.V., B.O. Boehm, and M. Tary-Lehmann. 2002. Vaccination with tumor peptide in CpG adjuvant protects via IFN-? dependent CD4 cell immunity. J. Immunol. 168: 6099-6105.
  • Lonsdorf, A.S., H. Kuekrek, B.V. Stern, B.O. Boehm, P.V. Lehmann and M. Tary-Lehmann. 2003. Intratumor CpG-oligodeoxynucleotide injection induces protective antitumor T cell immunity. J. Immunol. 171: 3841-3946.
Transplantation Immunology
  • Lehmann, P.V., and P. Heeger. 1997. Induction of Th1 or Th2 immunity to immunodominant indirect pathway peptide. Transplantation. 64: 292-96.
  • Matesic, D., P.V. Lehmann, and P.S. Heeger. 1998. High resolution characterization of cytokine producing alloreactivity in naive and allograft primed mice. Transplantation. 65: 906-914.
  • Tary-Lehmann, M., D. Hricik, A. C. Justice, N. S. Potter and P.S. Heeger. 1998. Enzyme-linked immunosorbent assay spot detection of interferon-? and interleukin 5 producing cells as a predictive marker for renal allograft failure. Transplantation. 66: 219-224.
  • Heeger, P.S., N. Greenspan, S. Kuhlenschmidt, C. Dejelo, D.E. Hricik, J. Schulak and M. Tary-Lehmann. 1999. Pretransplant frequency of donor specific, IFN-? producing lymphocytes is a manifestation of immunologic memory and correlates with the risk of posttransplant rejection episodes. J. Immunology. 163: 2267-2275.
  • Heeger, P.S., A. Valujskih and P.V. Lehmann. 2000. Comprehensive assessment of determinant specificity, frequency, and cytokine signature of the primed CD8 cell repertoire induced by a minor transplantation antigen. J. Immunol. 165: 1278-1284.
  • Gebauer, B.S., D.E. Hricik, A. Atallah, K. Bryan, J. Riley, M. Tary-Lehmann, N.S. Greenspan, B.O. Boehm, B.J. Hering and P.S. Heeger. 2002. Evolution of the Enzyme-Linked Immunosorbent Spot Assay for Post-Transplant Alloreactivity as a Potentially Useful Immunme Monitoring Tool. The American Journal of Transplantation. 9: 857-866.
  • Hricik, D.E., V. Rodriguez, R.N. Jocelyn Riley, K. Bryan, M. Tary-Lehmann, N. Greenspan, C. Dejelo, J.A. Schulak, and P.S Heeger. 2003. Enzyme linked immunosorbent spot (ELISPOT) assay for Interferon-? independently predicts renal function in kidney transplant recipients. Am. J. Transplantation. 7: 878-884.

1. Pau Lehmann的文献检索： NCBI
2. 美国凯西斯大学（西部保留地大学， Case Western Reserve Univ. ）的校方主页： CWRU
3. CWRU 主页： www.cwru.edu
4. CWRU 生物医学的全美排名： 2004 USNEWS 年美国大学排名

Copyright.(C) 美国细胞科技责任有限公司 中国办事处